ApoTome1 2nd floor inverse CRTD (Zeiss)
It is designed for routine work on fixed samples. Optical sectioning can be obtained on fixed samples by the use of the ApoTome1.
Category
Widefield, ApoTome
Location
CRTD, 2nd floor, LMF CMCB, room 2.230
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
2.5x/0.08 EC Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 9.5 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 120 2.5x/0.075 Zeiss |
| Order Number: | 440310-9903-000 |
10x/0.3 EC Plan-Neofluar, Air, Ph1, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 5.2 mm |
| Nomarski Prism: | EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 143 10x/0.3 Ph1 Zeiss |
| Order Number: | 420341-9911-000 |
20x/0.8 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.55 mm |
| Nomarski Prism: | EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 146 20x/0.8 Zeiss |
| Order Number: | 420650-9901-000 |
40x/0.95 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.25 mm |
| Nomarski Prism: | EC PN and Fluar 40/1,3 III; PA 40/0,95 III (426944) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 168 40x/0.95 Zeiss |
| Order Number: | 420660-9970-000 |
40x/1.3 EC Plan-Neofluar, Oil, DIC, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.21 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 144 40x/1.3 Oil Zeiss |
| Order Number: | 420460-9900-000 |
63x/1.4 Plan-Apochromat, Oil, DIC, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.19 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 145 63x/1.4 Oil Zeiss |
| Order Number: | 420780-9900-000 |
Condenser
Fluorescence Filter Cubes
motorized revolver
DAPI (set 49)
single cube for Alexa 350, DAPI
- BP 387/11 (BP 381-392), excitation filter, bandpass, Zeiss
- LP 395, dichromatic mirror, longpass, Zeiss
- BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
Zeiss, set 49
GFP (set 38 HE)
single cube for Alexa488/GFP, FITC
- BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
- LP 495, dichromatic mirror, longpass, Chroma
- BP 525/50 (BP 500-550), emission filter, bandpass, Chroma
Zeiss, set 38 HE
Cy3 (set 43 HE)
single cube for Alexa555/Cy3/RFP, TRITC
- BP 550/25 (BP 525-575), excitation filter, bandpass, Zeiss
- LP 570, dichromatic mirror, longpass, Zeiss
- BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss
Zeiss, set 43 HE
Cy5 (set 49006)
single cube for Alexa633/Cy5, Cy5
- BP 620/60 (BP 590-650), excitation filter, bandpass, Chroma
- LP 660, dichromatic mirror, longpass, Zeiss
- BP 700/75 (BP 663-738), emission filter, bandpass, Zeiss
Zeiss, set 49006
polarizer (set Analysator)
polarizer cube for DIC/Polarization
Zeiss, set Analysator
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
HXP 120C
Zeiss, Metal halide lamp, 120W, manual
Camera
Zeiss AxioCam MRm, Monochrome CCD camera, dexel size 6.45 µm
| port | left side port |
| magnification | 1x |
| bit depth | 12 bit |
| sensor size | 8.9 x 6.7 mm |
| maximum frame size | 1388 x 1040 dexel |
| maximum full chip frame rate | 12.86 fps |
XY Stage
Structured Illumination
ApoTome1, Zeiss
structured illumination for optical sectioning
Computer
Software
Additional Equipment
Hier kann weiteres zum System verfügbares equipment eingetragen werden.
Additional Information