Live Cell 4D BIOTEC (Zeiss)
Our Zeiss Axiovert 200 4D-System is designed for rapid acquisition LiveCell imaging in XYZ. The incubator allows control on temperature and CO2, and the motorized stage allows multiple-position imaging. Objectives can be mounted on a piezo for very fast z-acquisition.
Category
Widefield
Location
BIOTEC, 2nd floor, LMF CMCB, room 226
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
5x/0.15 EC Plan-Neofluar, Air, Ph1, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 13.6 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 138 5x/0.15 Ph1 Zeiss |
| Order Number: | 440321-9901-000 |
10x/0.3 EC Plan-Neofluar, Air, Ph1, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 5.2 mm |
| Nomarski Prism: | EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 108 10x/0.3 Ph1 Zeiss |
| Order Number: | 440331-9902-000 |
10x/0.45 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2 mm |
| Nomarski Prism: | Planapo 10/0,45 II (1045-073) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 121 10x/0.45 Zeiss |
| Order Number: | 000000-1063-139 |
20x/0.3 EC Plan-Neofluar, Air, Ph2, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2 mm |
| Nomarski Prism: | EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 41 20x/0.5 Ph2 Zeiss |
| Order Number: | 440341-9904-000 |
20x/0.8 Plan-Apochromat, Air, Ph2, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.55 mm |
| Nomarski Prism: | EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 141 20x/0.8 Ph2 Zeiss |
| Order Number: | 440641-9911-000 |
20x/0.8 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.55 mm |
| Nomarski Prism: | EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 171 20x/0.8 Zeiss |
| Order Number: | 440640-9903-000 |
40x/0.6 LD Plan-Neofluar, Air, DIC, Zeiss
| Immersion: | Air |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2.9 mm at cover glass 0.75 mm |
| Nomarski Prism: | LD PN 40/0,60 II (426955), LD PN 40/0,6 (444505) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 193 LD 40x/0.6 Zeiss |
| Order Number: | 421360-9970-000 |
40x/0.6 LD Plan-Neofluar, Air, Ph2, Zeiss
| Immersion: | Air |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2.9 mm |
| Nomarski Prism: | LD PN 40/0,60 II (426955), PlasDIC for LD PN 40/0,60 Korr (444505) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 36 LD 40x/0.6 Ph2 Zeiss |
| Order Number: | 441351-9970-000 |
40x/1.3 Plan-Neofluar, Oil, DIC, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.21 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 94 40x/1.3 Oil Zeiss |
| Order Number: | 1056-602 (1083-997) |
40x/1.3 EC Plan-Neofluar, Oil, Ph3, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.2 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 95 40x/1.3 Oil Ph3 Zeiss |
| Order Number: | 440451-9903-000 |
20x/0.75 EC Plan-Neofluar, Air, Ph2, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.71 mm |
| Nomarski Prism: | EC PN 40/0,75 II; 100/1,30III (426945) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 100 40x/0.75 Ph2 Zeiss |
| Order Number: | 440351-9903-000 |
63x/1.4 Plan-Apochromat, Oil, Ph3, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.19 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 43 63x/1.4 Oil Ph3 Zeiss |
| Order Number: | 1056-602 (1083-997) |
63x/1.2 C-Apochromat, Water, DIC, Zeiss
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.28 mm |
| Nomarski Prism: | EC PN 63/1,25 III; CA 63/1,2W III (426946), EC PN HC 63/1,25 II; CA HC 63/1,20 W II (426950) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 44 63x/1.2 Water Zeiss |
| Order Number: | 441777-9970-000 |
100x/1.4 Plan-Apochromat, Oil, DIC, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.17 mm |
| Nomarski Prism: | PA 100/1,4 HC II (426949), PA 40x/1,30 III (426965) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 39 100x/1.4 Oil Zeiss |
| Order Number: | 000000-1113-108 |
Condenser
Fluorescence Filter Cubes
motorized revolver
DAPI ()
single cube for Alexa 350, DAPI
- BP 360/40 (BP 340-380), excitation filter, bandpass, Leica
- LP 400, dichromatic mirror, longpass, Leica
- BP 460/50 (BP 435-485), emission filter, bandpass, AHF
Zeiss
GFP (set 49002)
single cube for Alexa488/GFP, FITC
- BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
- LP 495, dichromatic mirror, longpass, Chroma
- BP 525/50 (BP 500-550), emission filter, bandpass, Chroma
Zeiss, set 49002
GFP (set 49002)
single cube for Alexa488/GFP, FITC
- LP 405, dichromatic mirror, longpass
- BP 510/40 (BP 490-530), emission filter, bandpass
Zeiss
GFP (set F36-497)
single cube for Alexa488/GFP, FITC
- BP 472/30 (BP 457-487), excitation filter, bandpass, AHF
- LP 495, dichromatic mirror, longpass, AHF
- LP 496, emission filter, longpass, AHF
Zeiss, set F36-497
Texas red (set F46-008)
single cube for mCherry/TexasRed, DsRed
- BP 560/40 (BP 540-580), excitation filter, bandpass, AHF
- LP 585, dichromatic mirror, longpass, AHF
- BP 630/75 (BP 593-668), emission filter, bandpass, AHF
Zeiss, set F46-008
Cy5 (set 49006)
single cube for Alexa633/Cy5
- BP 620/60 (BP 590-650), excitation filter, bandpass, Chroma
- LP 660, dichromatic mirror, longpass, Chroma
- BP 700/75 (BP 663-738), emission filter, bandpass, Chroma
Zeiss, set 49006
GFP/RFP (set 59022)
double cube for GFP/RFP
- BP 470/40; BP 572/35 (BP 450-490; BP 555-590), excitation filter, double bandpass, Chroma
- BP 522/52, 637/83 (BP 496-548, 595-678), double dichromatic mirror, double bandpass, Chroma
- BP 522/41; BP 632/62 (BP 501-542; BP 601-663), emission filter, double bandpass, Chroma
Zeiss, set 59022
CFP/YFP (set 59217)
double cube for CFP/YFP
- BP 430/24; BP 500/20 (BP 418-442; BP 490-510), excitation filter, double bandpass, Chroma
- BP 433/22, 500/18 (BP 422-444, 492-510), double dichromatic mirror, double bandpass, Chroma
- BP 469/22; BP 535/30 (BP 458-480; BP 520-550), emission filter, double bandpass, Chroma
Zeiss, set 59217
calcium imaging (set 59217)
double cube for Calcium Imaging
- BP 340/12; BP 380/14, excitation filter, double bandpass
- LP 405, dichromatic mirror, longpass
- BP 510/40, emission filter, bandpass
Zeiss
Fura ratio imaging (calcium) (set F76-520)
double cube for Calcium Imaging
- BP 340/26 (BP 327-353), excitation filter, bandpass, AHF
- BP 387/11 (BP 385-392), excitation filter, bandpass, AHF
- LP 409, dichromatic mirror, longpass, AHF
- BP 510/84 (BP 468-552), emission filter, bandpass, AHF
Zeiss, set F76-520
polarizer (set Analysator)
polarizer cube for DIC/Polarization
Zeiss, set Analysator
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
DG4
Sutter, Xenon lamp, integrated
Spectra X Light engine
Lumencor, LED, integrated
Camera
Hamamatsu Orca Flash 4.0 V3, Monochrome sCMOS camera, dexel size 6.5 µm
| port | left side port |
| magnification | 1x |
| bit depth | 16 bit |
| sensor size | 13.312 x 13.312 mm |
| maximum frame size | 2048 x 2048 dexel |
| maximum full chip frame rate | 100 fps |
| Serial number | 300050 |
XY Stage
Incubation
Computer
Additional Information