LSM 780 upright CRTD (Zeiss)
It is an upright Laser Scanning Confocal microscope with 32 spectral detection channels (GaAsP), 2 PMTs, one Airyscan-detector and one transmission PMT. A selection of objective lenses is listed below, but more are available, too.
Suitable for the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, spectral unmixing of overlapping emission spectra, Airyscan super resolution. It can acquire brightfield and DIC images with the transmission detector.
Category
Laser scanning confocal
Location
CRTD, basement south, LMF CMCB, room -1.123
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
10x/0.45 C-Apochromat, Water, Zeiss
| Immersion: | Water |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 1.8 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 34 10x/0.45 Water Zeiss |
| Order Number: | 440638-9901-000 |
10x/0.45 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2 mm |
| Nomarski Prism: | Planapo 10/0,45 II (1045-073) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 124 10x/0.45 Zeiss |
| Order Number: | 420640-9900-000 |
20x/0.8 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.55 mm |
| Nomarski Prism: | EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 125 20x/0.8 Zeiss |
| Order Number: | 420650-9901-000 |
25x/0.8 LD LCI Plan-Apochromat, W / Si / Glyc / Oil, DIC, Zeiss
| Immersion: | W / Si / Glyc / Oil |
| Collar: | immersion correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.57 mm at cover glass 0.17 mm |
| Nomarski Prism: | LCI PN 25/0,8 II (426947) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 181 LD 25x/0.8 Imm Zeiss |
| Order Number: | 420852-9871-000 |
40x/1.2 C-Apochromat, Water, DIC, Zeiss
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.28 mm |
| Nomarski Prism: | CA 40/1,2 W; LD CA 40/1,1 W III (426948) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 128 40x/1.2 Water Zeiss |
| Order Number: | 421767-9970-000 |
40x/1 W Plan-Apochromat, Water, DIC, Zeiss
| Immersion: | Water |
| Working Distance: | 2.5 mm |
| Nomarski Prism: | W PA 40/1,0 III (426967-9010) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 132 40x/1.0 Water dipping Zeiss |
| Order Number: | 421462-9900-000 |
40x/1.4 Plan-Apochromat, Oil, DIC, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.13 mm |
| Nomarski Prism: | CA 40/1,2 W; LD CA 40/1,1 W III (426948) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 134 40x/1.4 Oil Zeiss |
| Order Number: | 420762-9900-000 |
100x/1.46 alpha Plan-Apochromat, Oil, DIC, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.11 mm |
| Nomarski Prism: | PA 100/1,46 III (426963) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 161 100x/1.46 Oil Zeiss |
| Order Number: | 420792-9800-000 |
5x/0 APO, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 137 APO Calibration Zeiss |
Condenser
Fluorescence Filter Cubes
motorized revolver
DAPI (set 49)
single cube for Alexa 350, DAPI
- BP 347/55 (BP 320-375), excitation filter, bandpass, Zeiss
- LP 395, dichromatic mirror, longpass, Zeiss
- BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
Zeiss, set 49
GFP (set 38)
single cube for Alexa488/GFP, FITC
- BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
- LP 495, dichromatic mirror, longpass, Chroma
- BP 525/50 (BP 500-550), emission filter, bandpass, Chroma
Zeiss, set 38
Cy3 (set 43)
single cube for Alexa555/Cy3/RFP, TRITC
- BP 545/25 (BP 533-558), excitation filter, bandpass, Zeiss
- LP 570, dichromatic mirror, longpass, Zeiss
- BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss
Zeiss, set 43
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
HXP 120C
Zeiss, Metal halide lamp, 120W, integrated
405nm
Laser Diode, integrated
458, 488, 514nm multiline
Laser Argon, integrated
561nm
Laser DPSS, integrated
594nm
Laser HeNe, integrated
633nm
Laser HeNe, integrated
XY Stage
Scan Head
Computer
Software
Additional Information