Biopolis Dresden Imaging Platform

DZNE M1 LSM 780 NLO (Zeiss)

Zeiss LSM 780 NLO

- Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.

- Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition.

Category

Laser scanning confocal, Multi photon

Location

DZNE, LMF DZNE, room 252

Suitable for Specimen
Fixed samples
Cleared samples
Whole organs
Tissue sections
Suitable for Technique
Spectral unmixing
3D imaging
Microscope Stand

Zeiss Axio Examiner.Z1, upright stand
manual objective revolver, motorized z-focus

Objectives

10x/0.45 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 2 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 06 10x/0.45 Zeiss
Order Number: 420640-9900

20x/0.8 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.55 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 07 20x/0.8 Zeiss
Order Number: 420650-9901

40x/0.95 Plan-Apochromat, Air, Zeiss

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.13-0.21
Working Distance: 0.25 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 08 40x/0.95 Korr Zeiss
Order Number: 420660-9970

10x/0.5 Clr Plan-Apochromat, Scale, Zeiss

Immersion: Scale
Working Distance: 3.7 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 09 10x/0.5 Clr Zeiss
Order Number: 421440-9800

20x/1 LD SC Plan-Apochromat, Scale, Zeiss

Immersion: Scale
Collar: immersion correction
Working Distance: 5.6 mm
Thread: M32
Tube Length: infinity
Objective ID: DZNE 10 20x/1.0 Clr Zeiss
Order Number: 421459-9770
Condenser

BF, DIC II, DIC III
NA 1.2 W, working distance 2 mm with 0.17mm cover glas and water immersion, manual

Fluorescence Filter Cubes

motorized revolver

GFP (set 38)

single cube for Alexa488/GFP, CFP, FITC

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss

Zeiss, set 38

Alexa 546 (set 38)

single cube for Alexa 546, TRITC, Cy3, DsRed

  • BP 545/25 (BP 520-570), excitation filter, bandpass, Zeiss
  • LP 570, dichromatic mirror, longpass, Zeiss
  • BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss

Zeiss, set 38

DAPI (set 488049-9901-000)

single cube for DAPI

  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss

Zeiss, set 38, set 488049-9901-000

Additional Filters

Cy5, DAPI, GFP, RFP

  • BP 700/75 (BP 663-738), emission filter, bandpass, Zeiss
  • BP 447/60 (BP 417-477), emission filter, bandpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss
  • BP 592/35 (BP 575-610), emission filter, bandpass, Zeiss
  • LP 488, dichromatic mirror, longpass, Zeiss
  • LP 560, dichromatic mirror, longpass, Zeiss
  • LP 643, dichromatic mirror, longpass, Zeiss
Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HXP 120C

Zeiss, Metal halide lamp, 120W, manual

405nm

Laser Diode, integrated

633nm

Laser HeNe, integrated

561nm

Laser DPSS, integrated

458, 488, 514nm multiline

Laser Argon, manual

680-1080 nm

Coherent, Laser Multiphotonen Chameleon, manual
XY Stage

motorized stage

Zeiss DC MCU 2008 with

  • variable holder for slides and 3.5cm dishes
Scan Head
Additional Information
  • Laser power values
  • See beam path
  • SIP Number 250400174
  • Contact for Service Daniel Sommer d.sommer@zeiss.de 015127782149
  • Service Contract Number PO 4500045803 (Zeiss) and 256148 (Coherent)
  • DRESDEN concept ID 2037
  • Date of Installation 19.04.2011
  • End of Warranty 19.04.2013