Biopolis Dresden Imaging Platform

DZNE W1 Zeiss ApoTome (Zeiss)

Structured Illumination Microscope

It is designed for routine work on fixed samples. Optical sectioning can be obtained on fixed samples by the use of the Apotome.

Category

Widefield, ApoTome

Location

DZNE, LMF DZNE, room 157

Suitable for Specimen
Fixed samples
Tissue sections
Suitable for Technique
Large area tile/mosaic scans
Microscope Stand

Zeiss Axio Imager.M2, upright stand
motorized objective revolver, motorized z-focus

Objectives

5x/0.16 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 12.1 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 01 5x/0.16 Zeiss
Order Number: 420630-9900

10x/0.45 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 2 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 02 10x/0.45 Zeiss
Order Number: 420640-9900

20x/0.8 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.55 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 03 20x/0.8 Zeiss
Order Number: 420650-9901

40x/0.95 Plan-Apochromat, Air, Zeiss

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.13-0.21
Working Distance: 0.25 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 04 40x/0.95 Korr Zeiss
Order Number: 420660-9970

100x/1.3 EC Plan-Neofluar, Oil, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.2 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 05 100x/1.3 Zeiss
Order Number: 420490-9900
Condenser

BF, DIC, Ph1, Ph2, Ph3
NA 0.9, working distance 1.2 mm, manual

Fluorescence Filter Cubes

motorized revolver

Alexa 555 ()

single cube for Alexa 555, TRITC

  • BP 546/12 (BP 540-552), excitation filter, bandpass, Zeiss
  • LP 560, dichromatic mirror, longpass, Zeiss
  • BP 607/65 (BP 575-640), emission filter, bandpass, Zeiss

GFP (set 489038-9901-000)

single cube for Alexa488/GFP, CFP, FITC

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss

, set 489038-9901-000

DAPI (set 488049-9901-000)

single cube for DAPI

  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss

, set 489038-9901-000, set 488049-9901-000

Cy5 (set 488049-9901-000)

single cube for Alexa633/Cy5

  • BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss

, set 489038-9901-000, set 488049-9901-000

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HXP 120C

Zeiss, Metal halide lamp, 120W, integrated
Camera

Zeiss AxioCam MRm, Monochrome CCD camera, dexel size 6.45 µm

port top port
magnification 1x
bit depth 12 bit
sensor size 8.9 x 6.7 mm
maximum frame size 1388 x 1040 dexel
maximum full chip frame rate 12.86 fps
Serial number 111114685

Zeiss AxioCam 503 color, Color CCD camera, dexel size 4.54 µm

port left side port
magnification 1x
bit depth 14 bit
sensor size 8.7 x 6.6 mm
maximum frame size 1936 x 1460 dexel
maximum full chip frame rate 38 fps
Inventory number 30085350
Serial number 214140105
XY Stage

motorized stage

Zeiss 130x85 STEP (D) with

  • holder for 1 slide
Structured Illumination

ApoTome2, Zeiss

structured illumination for optical sectioning

Additional Information
  • Inventory Number 8000015
  • SIP Number 3525000594
  • Contact for Service Raik Staeber raik.staeber@zeiss.com
  • Service Contract Number Po 4500045803
  • DRESDEN concept ID 2036
  • Date of Installation 01.01.1970
  • End of Warranty 01.01.1970