Biopolis Dresden Imaging Platform

CO1 - Olympus Fluoview 1000 (OLYMPUS)

Single photon point scanning, Olympus FV1000 confocal microscope with four confocal PMTs and one transmitted light detector. Inverted configuration with additional scanner and pulsed picosecond laser.

Location

MPI-CBG, LMF MPI-CBG, room 038A

Suitable for Specimen
Fixed samples
Live samples
Suitable for Technique
Photo manipulation (PA/FRAP/FLIP/FLAP/...)
Laser cutting/ ablation
Multi wellplate imaging
Microscope Stand

OLYMPUS IX 81, inverted stand
motorized objective revolver, motorized z-focus

Additional magnification: 1/1.6x

Objectives

10x/0.4 U Plan SApo, OLYMPUS

Coverslip Thickness: 0.17
Working Distance: 3.1 mm
Nomarski Prism: A
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 15 10x/0.4 Olympus
Order Number: 11506038

20x/0.85 U Plan SApo, Oil, OLYMPUS

Immersion: Oil
Working Distance: 0.17 mm
Nomarski Prism: A
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 16 20x/0.85 Olympus
Order Number: UPLSAPO20XO

40x/0.9 U Plan SApo, Air, OLYMPUS

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.11-0.23
Working Distance: 0.18 mm
Nomarski Prism: A
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 17 40x/0.9 Olympus
Order Number: UPLSAPO40X
Inventory Number: 4064380000

40x/0.65 U Apo, Oil, OLYMPUS

Immersion: Oil
Collar: NA correction
Coverslip Thickness: 0.17
Working Distance: 0.1 mm
Nomarski Prism: A
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 18 40x/1.35-0.65 Olympus
Order Number: UAPON 40XO340-2

60x/1.2 U Plan SApo, Water, OLYMPUS

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.13-0.21
Working Distance: 0.28 mm
Nomarski Prism: A
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 19 60x/1.2 Olympus
Order Number: UPLSAPO60XW

60x/1.35 U Plan SApo, Oil, OLYMPUS

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.15 mm
Nomarski Prism: A
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 20 60x/1.35 Olympus
Order Number: UPLSAPO60XO
Condenser

BF, DIC
NA 0.55, motorized

Fluorescence Filter Cubes

motorized revolver

GFP ()

single cube for Alexa488/GFP, FITC

  • BP 475/30 (BP 460-490), excitation filter, bandpass, OLYMPUS
  • LP 500, dichromatic mirror, longpass, OLYMPUS
  • LP 520, emission filter, longpass, OLYMPUS

RFP ()

single cube for RFP, Alexa 568

  • BP 530/40 (BP 510-550), emission filter, bandpass, OLYMPUS
  • LP 570, dichromatic mirror, longpass, OLYMPUS
  • LP 590, excitation filter, longpass, OLYMPUS

DAPI ()

single cube for DAPI, Alexa 350

  • BP 330/54 (BP 330-385), excitation filter, bandpass, OLYMPUS
  • LP 400, dichromatic mirror, longpass, OLYMPUS
  • LP 420, excitation filter, longpass, OLYMPUS

polarizer ()

single cube for DAPI, Alexa 350

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

X-Cite 120W

X-Cite, Metal halide lamp

458, 477, 488, 514nm multiline

Laser Argon, manual

375nm

Laser Diode, integrated

405nm

Laser Diode, manual

405nm pulsed

Laser Diode, manual

561nm

Laser DPSS, manual

633nm

Laser HeNe, manual
XY Stage

motorized stage

Prior with

  • heating insert P-Set 2000 (Pecon)
  • multiwell plate holder
  • holder for 1 slide
  • petri dish holder
Scan Head

Fluoview FV1000

conventional scanner

Internal detectors:

  • 2 confocal PMT (filter)
  • T-PMT
Extra Photo Manipulation

Scanner

Fluoview FV1000 ASU

Photo Manipulation light source:

Picoquant laser module
     355 ps pulsed laser
     375 ps pulsed laser
     405 ps pulsed laser

Incubation

stage top incubator

Pecon

Feature:

  • Heating

  • CO2

Additional Information
  • Laser power values
  • Inventory Number MOZG0407421000
  • SIP Number 6E08
  • Contact for Service Dirk Hohmann
  • DRESDEN concept ID 1271
  • Date of Installation 31.08.2005