Biopolis Dresden Imaging Platform

W2 - DeltaVision Elite !! Retired !! (GE)

Wide-Field Microscope

High end widefield fluorescence / transmitted light (DIC) microscope with built in deconvolution. New generation motorised stage, Ultimate focus hardware autofocus system, and environmental control.

Suitable for Live cell imaging, time lapse, multi point, multi-well plates. Tile scanning large samples. Scanning multi-well plates; High resolution deconvolution fluorescence microscopy..

Category

Widefield

Location

MPI-CBG, LMF MPI-CBG, room U35

Suitable for Specimen
Fixed samples
Live samples
Suitable for Technique
Multi wellplate imaging
Microscope Stand

GE IX 71, inverted stand
motorized objective revolver, motorized z-focus

Additional magnification: Optovar 1x / 1.6x, manual

Objectives

10x/0.4 U Plan SApo, Air, OLYMPUS

Immersion: Air
Coverslip Thickness: 0.17
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 355 10x/0.4 Olympus
Order Number: UPLSAPO10X2
Inventory Number: 355

20x/0.75 U Plan SApo, Air, OLYMPUS

Immersion: Air
Coverslip Thickness: 0.17
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 356 20x/0.75 Olympus
Order Number: UPLSAPO20X

40x/1.3 U Plan Fl, Oil, OLYMPUS

Immersion: Oil
Coverslip Thickness: 0.17
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 357 40x/1.3 Oil Olympus
Order Number: UPLFLN40XO

60x/1.42 Plan Apo N, Oil, OLYMPUS

Immersion: Oil
Coverslip Thickness: 0.17
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 358 60X/1.42 Oil Olympus
Order Number: PLAPON60XO

60x/1.2 Plan Apo S, Water, OLYMPUS

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.13-0.21
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 359 60X/1.2 W Olympus
Order Number: UPLSAPO60XW

100x/1.4 U Plan SApo, Oil, OLYMPUS

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.13 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 176 100x/1.4 Olympus
Order Number: UPLSAPO100XO
Condenser

BF, DIC I, DIC II, DIC III
NA 0.55

Fluorescence Filter Cubes

motorized revolver

DAPI ()

single cube for DAPI

  • BP 435/48, emission filter, bandpass

FITC ()

single cube for FITC

  • BP 525/36, emission filter, bandpass

TRITC ()

single cube for TRITC

  • BP 594/45, emission filter, bandpass

Cy5 ()

single cube for TRITC

  • BP 676/34, emission filter, bandpass

EMPTY ()

empty for through position

  • empty

polarizer ()

empty for through position

CFP ()

single cube for through position

  • BP 470/24, emission filter, bandpass

YFP ()

single cube for through position

  • BP 535/30, emission filter, bandpass

GFP ()

single cube for through position

  • BP 525/30, emission filter, bandpass

mCherry ()

single cube for through position

  • BP 632/60, emission filter, bandpass

Additional Filters

DAPI, FITC, TRITC, Cy5, GFP, mCherry, CFP, YFP, DAPI, FITC, Alexa 594, Cy5

  • dichromatic mirror, bandpass
  • dichromatic mirror, bandpass
  • dichromatic mirror, bandpass
  • dichromatic mirror, bandpass

BFP, DAPI, Alexa 350, CFP, GFP, eGFP, Alexa 488, FITC, DsRed, RFP, TRITC, Cy3, Alexa 555, Alexa 568, Alexa 568, Alexa 594, Cy5, Alexa 647

  • (BP 381-399), excitation filter, bandpass
  • (BP 426-450), excitation filter, bandpass
  • (BP 461-489), excitation filter, bandpass
  • (BP 529-556), excitation filter, bandpass
  • (BP 563-588), excitation filter, bandpass
  • (BP 621-643), excitation filter
Transmitted Illumination

LED (LED)

Fluorescence Illumination

SSI Lumencor

Lumencor
Camera

Photometrics, Monochrome EMCCD camera, dexel size 16 µm

port left side port
bit depth 16 bit
sensor size 8.2x8.2 mm
maximum frame size 512 x 512 dexel
maximum full chip frame rate 20.4 fps
Serial number 53853054001

PCO edge, Monochrome sCMOS camera, dexel size 6.5 µm

port left side port
bit depth 15 bit
sensor size 16.6x14 mm
maximum frame size 2560 x 2160 dexel
maximum full chip frame rate 200-400 fps
Serial number 53853043001
Hardware Autofocus

GE Ultimate focus

Incubation

cage incubator

GE

Feature:

  • Heating

  • CO2

Additional Information