Biopolis Dresden Imaging Platform

Ultramicroscope CRTD (LaVisionBioTec)

Single Plane Illumination microscope

Static light sheet microscope with 2 light sheets. Each sheet is composed out of three individual sheets from different angles to reduce shadowing. The sample can be moved about 1cm3 in xyz. Due to the white light laser/excitation/emission filter wheels a broad range of fluorophores can be used.

Suitable for 3D multicolor imaging of large transparent specimen.

Category

Light sheet

Location

CRTD, ground floor south, LMF CMCB, room -1.120

Suitable for Specimen
Cleared samples
Microscope Stand

LaVisionBioTec MVX10, upright stand
manual objective revolver, motorized z-focus

Additional magnification: 10x optical zoom, motorized

Objectives

2x/0.5 MVPLAPO, Air, OLYMPUS

Immersion: Air
Coverslip Thickness: -
Working Distance: 20 mm
Objective ID: CMCB 194 2x/0.5 Olympus
Additional Filters

DAPI, Alexa 350, Alexa 405, Alexa 488, FITC, GFP, YFP, Cy3, TRITC, Cy5, DsRed, Texas Red, Cy7

  • BP 405/40 (BP 385-425), excitation filter wheel, bandpass
  • BP 470/40 (BP 450-490), excitation filter wheel, bandpass
  • BP 500/20 (BP 490-510), excitation filter wheel, bandpass
  • BP 545/60 (BP 515-575), excitation filter wheel, bandpass
  • BP 560/40 (BP 540-580), excitation filter wheel, bandpass
  • BP 620/40 (BP 600-640), excitation filter wheel, bandpass
  • BP 740/35 (BP 723-758), excitation filter wheel, bandpass

DAPI, Alexa 350, Alexa 405, Alexa 488, FITC, GFP, YFP, Cy3, TRITC, Cy5, DsRed, Texas Red, Cy7

  • BP 460/50 (BP 435-485), emission filter wheel, bandpass
  • BP 525/50 (BP 500-550), emission filter wheel, bandpass
  • BP 535/30 (BP 520-550), emission filter wheel, bandpass
  • BP 600/60 (BP 570-630), emission filter wheel, bandpass
  • BP 630/75 (BP 593-668), emission filter wheel, bandpass
  • BP 680/60 (BP 650-710), emission filter wheel, bandpass
  • BP 795/50 (BP 770-820), emission filter wheel, bandpass
Fluorescence Illumination

Laser Fianium Supercontinuum SC400-4 - range 410 to 2400 nm

Fianium, Laser, > 4 W, integrated
Camera

Andor Neo sCMOS, Monochrome sCMOS camera, dexel size 6.5 µm

port top port
sensor size 16.4 x 14 mm
maximum frame size 2560 x 2160 dexel
Computer

Windows 7 operating system

  • 16 GB RAM
  • Intel Core i5 CPU
  • 30" display
  • LVBT-PC
Software

LaVisionBioTec ImSpektor,

Additional Equipment

there are 2 different dipping caps available

  • short version - longer working distance of 7 mm but less resolution
  • long version - shorter working distance and higher resolution

Additional Information
  • SIP Number UM-0030
  • Contact for Service Herr Markus Boettcher, Tel. 0521-915139-27, boettcher@lavision.com
  • DRESDEN concept ID 1410
  • Date of Installation 05.03.2014